Coding

Part:BBa_K4187013:Design

Designed by: Martin Pezous   Group: iGEM22_Ionis_Paris   (2022-10-07)


Polyhydroxyalkanoate synthase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 87
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1060
    Illegal NgoMIV site found at 1339
    Illegal AgeI site found at 1167
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Has been mutated to reduce the GC% and allow synthesis.


Source

Pseudomonas sp. 61-3

References